[求救] co-culture
有幾個關於co-culture的問題.
看過幾篇期刊之後加上自己的想像.
Effects of neridronic acid on osteoclasts derived by physiological dual-cell cultures
http://0rz.tw/2d33C
Morphological features of osteoclasts derived from a co-culture system
http://www.springerlink.com/content/m030101131507xg8/
我要養macrophage和osteoblast.
自己認為只要分別培養到細胞數目達到一個標準.
即可將macrophage加入osteoblast中共同培養.
請教有做過co-culture的人.
得知會用到transwell.
推翻了我的假想.
想請問co-culture一定要用transwell?
可否單純一起養在dish中?
另外co-culture有沒有需要特別注意的地方?
麻煩大家幫我解答>"< 謝謝!!
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