[求救] mutation的問題
最近用quikchange的kit在做mutation
PCR之後有跑gel,可以看到明顯的band
DpnI digest之後做transform
卻沒有colony長出來 QQ
請問可能是哪步驟出問題了嗎??
請大家給我意見吧!!
謝謝~~
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