作者查詢 / chiehdis
作者 chiehdis 在 PTT [ Biotech ] 看板的留言(推文), 共22則
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6F推: protoplast, nucleus freezing, 電穿孔, agrobacteria03/26 21:14
8F推:用1/3體積的氯化鋰沉澱~~04/01 15:06
4F推:還有剩嘛????10/20 15:58
1F推:受益良多 感謝您!!!!10/01 14:56
3F推:因為我的實驗也有用到LiCl 所以並不是TRIzol去DNA的效果09/30 03:26
4F→:好!!是LiCl的功效嗎????? 天啊!!(震驚!!)09/30 03:27
5F→:我用氯化鋰主要是用來去多醣的~~09/30 03:29
1F推:就是你!!回我文的強者大!!!!09/29 05:31
1F推:天啊又來一個強者!!讓我獲益良多~不過或許我和原po需求09/28 23:33
2F→:不同 基本上我是要量大 NANO漂亮 28s/18s不降解09/28 23:34
3F→:可能我不太懂他的後續實驗設計(我沒做到啊!!囧~) 所以野09/28 23:35
4F→:人獻曝了一下~>"< 感謝你的回文!!!09/28 23:36
5F→:想另外請教 若NANO都ok~ 變性膠和非變性膠體都看不到gDNA09/28 23:38
6F→:(28s和1Kb ladder上都沒條帶) 這樣怎麼看出RNA仍有汙染??09/28 23:39
3F→:樓下該不會是你學弟吧???XD09/28 23:39
4F→:這只是我過去的經驗啦~因為我的RNA不是用來qPCR的~09/28 23:40
5F→:所以當然以你實驗為主囉!!^^ 看起來好像下面那篇回文更能09/28 23:41
6F→:幫助到你呢~!!!09/28 23:41
1F推:非常非常感謝你!!!!!09/28 22:40
5F→:萬分感謝大家的意見 我會去查查這方面的資料!!08/26 11:19
8F→:給樓上L大 我有經過LiCl的步驟 但我是為了去除多醣~08/27 05:34
9F→:謝謝大家 有提到的我都會找資料看看的~再次感謝!!!!08/27 05:35
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